Jasmonic acid is one of the important phytohormones responding to abiotic and biotic injury.Detecting the acid is difficult because of the low content in plant material.12-oxo-phytodienoic acid is an important precursor in the biosynthesis ofjasmonic acid.In this research,we established an HPLC-MS/MS method to determine the content of these two target chemicals simultaneously in 4 types of plant materials.An external standard curve was used to calculate the content in plant matedal.The regression coefficiency factor of the standard curve reached 0.991 9 to 0.999 5.Spiked recovery was from 91.91％ to 101.56％.The method is sensitive and available;The limits of detection of two target chemicals were 3.218 and 34.129 pmol.This method was used to determinate the contents of these two target chemicals in 4 types of plant materials.%茉莉酸是植物体内重要的伤反应特异激素,含量极低,但作用较大.当植物受到机械伤害时,茉莉酸调控一系列应激反应.12-氧-植物二烯酸(OPDA)是茉莉酸合成途径中的重要前体,与茉莉酸有密切的关系.该研究建立了一种HPLC-MS/MS分析检测方法,可同时检测植物材料中茉莉酸及其前体OPDA含量.利用该方法建立的标准曲线具有良好的线性相关性,相关系数达0.991 9-0.999 5,回收率范围为91.91％-101.56％.该方法同时具有较好的检测灵敏度,2种化合物的最低检测限为3.218和34.129 pmol.研究结果说明该方法切实可靠且具有较好的适用性.利用该方法检测了4种不同植物样品中目标化合物的含量,结果表明该方法能够用于不同植物材料中这2种活性化合物含量的检测分析.
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