COMPLEX APPROACH FOR ISOLATION AND HPLC-MS DETERMINATION OF STEROIDAL GLYCOSIDES FROM PLANT MATERIAL AND SUSPENSION CELL CULTURE

摘要

For thousands of years, herbal medicines made from terrestrial plants were used by humans to prevent and treat diseases. Extraction is the crucial first step in the analysis of medical plants. Researches have shown that most of the therapeutic properties of D. deltoidea and T. terrestris are due to the presence of steroidal glycosides. The main objectives of this study were to develop a complete strategy of isolation and HPLC-MS determination of steroidal glycosides.