固相萃取-高效液相色谱法测定紫杉醇脂质纳米粒体外释放度

摘要

OBJECTIVE: To develop a solid phase extraction (SPE)-HPLC method for in vitro drug release of Paclitaxel lipid nanoparticles, and to investigate in vitro drug release of Paclitaxel lipid nanoparticles within 24 h. METHODS: The sample was purified with Cia solid phase extraction columns. The chromatographic analysis was performed on a phenomenex luna C18(250 mmx4.6 mm,5 pin) column with mobile phase consisted of methanol-water (80-20) at the flow rate of 1.0 mL.min-1. The detection wavelength was set at 227 nm. RESULTS: The linear range of paclitaxel was 2~12 ug.mL-1(r=0.999 3). The in vitro cumulative release of paclitaxel in 1 mol.L-1 sodium salicylate solution -PH 7.4 PBS reached about 104.9% in 24 h. The profile was described by first-class equation. CONCLUSION: Themethod is simple, sensitive and little interference, and it is suitable for the determination of in vitro drug release of sample.%目的:建立测定紫杉醇脂质纳米粒体外释放度的方法,考察紫杉醇脂质纳米粒24h的体外释放度.方法:采用固相萃取-高效液相色谱法.样品经C18固相萃取柱萃取,采用Phenomenex luna C18(250 mm×4.6 mm,5μm)色谱柱分离,流动相为甲醇-水(80:20),流速为1.0 mL· min-1,检测波长为227nm.结果:紫杉醇的检测浓度在2～12 μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.999 3),紫杉醇脂质纳米粒在水杨酸钠(1 mol·L-1)-PBS(pH 7.4)中24 h累积释放度为104.9％,其释放规律符合一级动力方程.结论:所建方法简便、灵敏,干扰小,可用于体外释放的检测.