黄芪当归合煎剂对急性肾损伤模型大鼠的保护作用研究

摘要

OBJECTIVE: To study prevetion and treatment effects of Astaragali Radix and Angelica sinensis decoction on acute renal injury in rats and its mechanism. METHODS: Acute kidney injury (acute renal injury, AKI) model was induced by left and right kidney occlusion-reperfusion. Rats were divided into sham operation (constant volume of normal saline), model group (constant volume of normal saline), Astaragali Radix and A. sinensis decoction high-dose, medium-dose and low-dose groups (18, 9, 4.5 g/kg). They were given medicine intragastrically 7d before operation, once a day, for continuous 7d, 30 min after reperfusion by gavage for 1 time. 24 h after reperfusion, the contents of Scr and BUN were determined, and the contents of MDA and GSH, the activity of SOD in renal tissue homogenate were also determined. Renal tubular injury score were calculated. RESULTS: Compared with sham operation group, the contents of Scr and BUN in rat serum increased significantly and the content of MDA also increased significantly, while the content of GSH and the activities of SOD decreased significantly. The renal tubular injury score increased significantly (P<0.01). Compared with model group, the contents of Scr and BUN in Astaragali Radix and A. sinensis decoction high-dose and medium-dose groups decreased significantly, and the content of MDA also decreased significantly; SOD activity increased significantly; renal tubular injury score decreased significantly(P<0.01 or P<0.05). CONCLUSION: The effects of Astaragali Radix and A. sinensis decoction on acute renal injury in rats may be related to the improvement of renal tubular function and regulation of antioxidant factor.%目的:研究黄芪当归合煎剂对急性肾损伤(AKI)模型大鼠的保护作用.方法:以夹闭大鼠左、右肾后再灌注方法复制AKI模型.实验分为假手术(等容生理盐水)、模型(等容生理盐水)与黄芪当归合煎剂高、中、低剂量(18、9、4.5 g/kg)组.于手术前7d灌胃给药,每天1次,连续7d,再灌注30 min后再灌胃给药1次.于再灌注24 h后测定大鼠血清中血清肌酐(Scr)、尿素氮(BUN)含量,大鼠肾组织匀浆中丙二醛(MDA)、谷胱甘肽(GSH)含量,超氧化物歧化酶(SOD)活性；计算肾小管损伤评分.结果:与假手术组比较,模型组大鼠血清Scr、BUN含量显著升高,肾组织匀浆MDA含量显著增加,GSH含量显著减少,SOD活性显著减弱,肾小管损伤评分显著增加(P＜0.01).与模型组比较,黄芪当归合煎剂高、中剂量组大鼠血清Scr、BUN含量显著降低,肾组织匀浆MDA含量显著减少,SOD活性显著增强,肾小管损伤评分显著减少(P＜0.01或P＜0.05).结论:黄芪当归合煎剂对AKI模型大鼠的防治作用可能与其改善肾小管功能、调节抗氧化因子有关.