Objective: To establish a fast quantitative detection method for tartrazine, sunset yellow, orange Ⅱ sodium salt and allura red in safflower by solid phase extraction-electrospray ionization-high performance ion mobility spectrometry (SPE-ESI-HPIMS).Methods: The pigments were extracted by 70% ethanol with ultrasonic treatment, and then a polyamide SPE column was used to remove the complex matrix interference in safflower.The purified sample was then dissolved in 90% methanol and analyzed under the optimized IMS parameters.Results: The detection time of all the pigments was less than 20 ms.The limit of detection of tartrazine, sunset yellow, orange Ⅱ sodium salt and allura red was 0.17 , 0.15 , 0.30 and 0.25 μg·ml-1 , respectively.All the pigments showed excellent linearity within the range of 0.5-20 μg·ml-1 (r＞0.990 0), the method recovery was 88.0%-98.9% , and the RSD was 1.5%-5.2% (n =6).Conclusion: The method is rapid, simple, highly sensitive and reproducible, and suitable for the rapid quantitative detection of illegal added staining substances in safflower.%目的: 建立固相萃取-电喷雾高效离子迁移谱(SPE-ESI-HPIMS)快速检测红花中柠檬黄、日落黄、金橙Ⅱ、诱惑红并进行定量测定的方法.方法: 采用70%乙醇超声提取色素,再经聚酰胺固相萃取柱除去红花中复杂的基质干扰,随后用90%甲醇溶解净化后的样品,最后在各色素优化好的IMS参数下进样检测.结果: 色素IMS检测时间均不到20 ms,柠檬黄、日落黄、金橙Ⅱ、诱惑红的检出限分别为0.17, 0.15, 0.30, 0.25 μg·ml-1,均在 0.5 ~20 μg·ml-1 范围内具有良好线性(r＞0.990 0),平均回收率为88.0% ~ 98.9%, RSD为1.5% ~ 5.2% (n=6).结论: 该方法快速、简便、灵敏度高,重现性好,适用于红花中多种非法染色物质的快速检测和定量分析.
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