口虾蛄总生物碱对人肝癌HepG2细胞抗肿瘤作用实验研究

摘要

Objective To extract and analyze the anti-tumor function of the total alkaloids of Squilla oratoria against the human hep-atoma HepG2 cell. Methods The total alkaloids of Squilla oratoria were extracted by acid extraction and alkaline deposition,then isolated by ethyl acetate;CCK-8 assay,plate clone formation assay,flow cytometry,were performed to measure the effect of the total alkaloids of Squilla oratoria on the human hepatoma HepG2 cell. Results The IC50 of cell viability on HepG2 cells after treated with S for 24 h,48 h,and 72 h was 746. 72 μg/mL,47. 58 μg/mL or 26. 32 μg/mL,respectively. After treated with S (0 μg/mL, 10 μg/mL,50 μg/mL and 100 μg/mL)for two weeks,the cloning efficiency of HepG2 cells was 56. 1%,35. 7%,14. 1% or 0,re-spectively;After treated with S for 48 h ,the human hepatoma HepG 2 cells in S stage was increased from 26. 14% to 40. 80%. Conclusion The total alkaloids of Squilla oratoria has an inhibitory effect on the cell growth in HepG2 cells through the Pathways of cell cycle arrest.%目的：探讨口虾蛄总生物碱对人肝癌HepG2细胞的抗瘤效果。方法口虾蛄酸提碱沉，继以乙酸乙酯萃取；人胆囊收缩素( CCK-8)法测定不同质量浓度的口虾蛄总生物碱对HepG2细胞增殖的抑制作用；平板克隆实验观察其对HepG2细胞克隆形成能力的影响；流式细胞术检测其刺激HepG2细胞的细胞周期变化。结果口虾蛄总生物碱作用于HepG2细胞24，48，72 h后，CCK-8法检测细胞活性的半抑制浓度（ IC50）分别为746.72，47.58，26.32μg/mL；口虾蛄总生物碱（0，10，50，100μg/mL）作用2周后，各组细胞克隆形成率分别为56.1%，35.7%，14.1%，0；口虾蛄总生物碱作用48 h后，HepG2细胞周期的S期比例由26.14%上升为40.80%。结论口虾蛄总生物碱能明显抑制HepG2细胞的生长，这可能通过阻滞细胞周期来实现。