异养小球藻油脂正己烷提取工艺优化

摘要

The effects of key parameters such as mass concentration of algae cell solution,crushing pres-sure,crushing times,extraction time,extraction temperature and dosage of extractant ( hexane) on the oil yield were studied so as to obtain the optimal oil extraction process from heterotrophic Chlorella by hex-ane. The results showed that the optimal oil extraction process was obtained as follows:mass concentration of alage cell solution 110 g/L,crushing pressure 100 MPa,crushing times 4 times,extraction time 5 h,ex-traction temperature 40℃, dosage of hexane 9 mL/g ( based on the mass of dry algae powder ) . Under these conditions,the oil yield could reach the highest about 55% after extraction for 5 times.%为确定最佳异养小球藻油脂正己烷提取工艺,对提取工艺中的关键参数如藻细胞溶液质量浓度、破碎压力、破碎次数、萃取时间、萃取温度、萃取剂正己烷添加量对油脂得率的影响进行了研究.结果表明,最佳油脂提取工艺条件为藻细胞溶液质量浓度110 g/L、破碎压力100 MPa、破碎次数4次、萃取时间5 h、萃取温度40℃、正己烷添加量9 mL/g(以藻粉干重计) ,在该条件下萃取5次,油脂得率最高,可以达到55%左右.