High-concentration chlorella culture method by heterotrophic culture (cancer culture)

摘要

The present invention relates to a method for producing chlorella by heterotrophic culture (cancer culture), which comprises culturing chlorella in a sterilized culture tank with chlorophyll and glucose such as glucose as main carbon source and ammonia, urea, or a compound thereof as a main nitrogen source The culture was optimized by adjusting the initial sugar concentration to 4 to 8% (W / V) and the inoculum amount to 4 to 10% (v / v) Wherein a high concentration of chlorella is produced by adding an additional sugar such that the residual sugar is 3 to 4% (W / V) at a point of 1 to 2% (W / V) Which can be industrially easily cultured.