Method for the production of biodiesel by Chlorella culture in two stages, from the autotroph to the heterotroph

摘要

A method to produce biodiesel through an autotrophic heterotrophic culture in two steps of Chlorella, characterized in that microalgae cells with a lipid content greater than 50% are used, obtained by autotrophic culture first and then heterotrophic culture, as raw material to produce biodiesel ; The method comprises the following steps: (1) cultivate autotrophically Chlorella; (2) concentrate the cells; (3) ferment in a heterotrophic manner; (4) collect and dry algae cells; (5) extract lipids from dried algae cells; and (6) produce biodiesel by esterification reaction.