改性大豆磷脂在脂质体制备中的应用研究

摘要

以大豆磷脂和聚乙二醇单甲醚2000为原料,制备了大豆磷脂酰乙醇胺-聚乙二醇单甲醚2000(SPE-MPEG2000),产物结构经1H NMR和IR进行了表征.采用薄膜分散法制备了含SPE-MPEG2000的空间稳定脂质体(SSL),同时制备不含SPE -MPEG2000的普通脂质体(CLs).通过TEM考察了两种脂质体的形态及粒度分布,测定了粒径及Zeta电位变化,采用紫外分光光度法测定了脂质体加乙醇后的吸光度变化及包封钙黄绿素后的包封率.结果表明:SSL粒子呈类球形,粒径分布较CLs均一且分散性较好;放置前后SSL的粒径及吸光度变化较小,包封率高于CLs,从而说明SPE-MPEG2000对脂质体有良好的稳定作用.%Soybean phosphatidylethanolamine -polyethylene glycol monomethyl ether 2000(SPE -MPEG2000)was synthesized from soybean phospholipid and polyethylene glycol monomethyl ether 2000, and its structure was characterized by 1H NMR and IR.Liposomes with or without SPE -MPEG2000 were prepared by thin film dispersed method.The morphology and particle distribution of the two lipo-somes were observed by TEM.The changes of particle size and Zeta potential were determined.The in-fluence of adding ethanol on absorbance of liposomes and encapsulation efficiency of calcein were meas-ured by UV Spectrophotometry.The results showed that the liposomes prepared with SPE -MPEG2000 (SSL)were globular in appearance,distributed evenly and dispersed well compared with liposomes with-out SPE-MPEG2000(CLs)in TEM.The particle size and absorbance of SSL changed little after place-ment.The encapsulation efficiency of calcein by SSL was higher than by CLs.It indicated that SPE -MPEG2000 had a good stabilizing effect on liposomes.