植物沥青中角鲨烯的富集及分离提纯研究

摘要

The enrichment process,separation and purification process of squalene from plant asphalt were studied.The squalene was enriched in plant asphalt by cold separation centrifugation and molecular distillation,then the effects of solvent polarity,adsorbent dosage and adsorption time on the content and yield of squalene were investigated.Based on single factor experiment,the separation and purification conditions were optimized by orthogonal experiment as follows:with acetone-petroleum ether (volume ratio 30∶70) as solvent,mass ratio of silica gel to sample 1∶ 1,mass ratio of acticarbon to sample 3 ∶ 1.Under the optimal conditions,the content and yield of squalene were 87.34％ and 88.69％,respectively.%研究了植物沥青中角鲨烯的富集及分离提纯工艺.通过冷析离心和分子蒸馏对植物沥青中角鲨烯进行一定量的富集后,考察了溶剂极性、吸附剂用量和吸附时间对提纯后的角鲨烯含量和收率的影响.在单因素实验的基础上,通过正交实验优化提纯工艺条件,得出最佳工艺条件为:采用丙酮与石油醚(体积比30∶70)作为溶剂,吸附剂的量分别为硅胶与样品质量比1∶1、活性炭与样品质量比3∶1.在最佳工艺条件下,角鲨烯的含量和收率分别达到了87.34％和88.69％.