Objective To investigate the effects of different culture methods in vitro mouse zona-free embryonic devel-opment. Methods In various stages of embryonic development rate,blastocyst rate and cell numbers of blastocysts de-veloped were measured as mWOW system (the modified “well of well” system), micro-drops single + group, micro-drops single and micro-drops group culture in vitro. The effects of different methods of were compared in vitro embryo development zona-free. Results Embryonic development rate,blastocyst rate and cell numbers of blastocysts developed were significantly higher in mWOW system culture than micro-drops single + group, micro-drops single and micro-drops group culture. Conclusion Compared with micro-drops single+group,micro-drops single and micro-drops group culture,mWOW system method is more suitable for zona-free cultured in vitro mouse embryos.%目的：探讨不同体外培养方法对小鼠去透明带胚胎发育的影响。方法以胚胎各阶段发育率、囊胚率和囊胚细胞数作为衡量指标，对比微滴单卵法、微滴群卵法、微滴单卵+群卵法、mWOW培养法(the modified “well of well”system)四种体外培养方法，探讨不同体外培养方法对去透明带胚胎发育的影响。结果 mWOW培养法、微滴单卵+群卵法、微滴单卵法这三种方法之间的发育率、囊胚率以及囊胚数均有显著差异(P<0.05)。结论mWOW法与其他三种方法相比，更适合作为体外培养去透明带小鼠8-细胞以后阶段的方法，以及用于体内移植小鼠胚胎的体外培养方法。
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