Positive effect of high voltage electrostatic field on the development of in vitro fertilived mouse embryos

摘要

In order to investigate the effects of a high voltage electrostatic field (HVESF) on the preimplantation development of an IVF mammalian embryo, we used the mouse model to attempt at understanding the effect that HVESF may benefit preimplantation development. The optimal dosage (field intensity and treatment time) was selected according to the proportion of 1 or 2-cell embryos which develop into blastocysts or hatched blastocyst during in vitro culture. Using this optimal dosage treatment on mouse embryos, the effect of HVESF at different cell stages on their developmental potential was also investigated. The results showed: the HVESF can significantly promote the preimplantation development of embryos in vitro, at the optimal dosage stimulation the proportion of blastocysts increased from 42.4percent to 67.5percent, hatched blastocyst increased from 20.8percent to 48.4percent when compared to controls, and there were singnificant differences between the optimal dosage group and the control group. Different sensitivities existed among the embryos at different cell stages for HVESF, to which the 1 or 2-cell stage embryos were significantly more sensitive than others. Follewing, we have provided, to our knowledge, the first report of application of HVESF on mammalian embryology.