Objective To study the expression of p35 and EBI3 mRNA in colorectal cancer and analysis its correlation with clinicopathological features. MethodsThe expression of p35 and EBI3 mRNA in 30 cases of colorectal cancer and their corresponding adjacent tissues, and 20 cases of normal tissues were detected by RT-PCR. And the relationships between the p35 and EBI3 gene mRNA expression and clinicopathological features, metastasis were analyzed.ResultsP35 and EBI3 mRNA were higher expression in colorectal cancer than cancer corresponding adjacent tissues and normal tissues, respectively. The expression of p35 and EBI3 mRNA were correlated with clinicopathological features, tissue types and lymph node metastasis, but not with age and gender. The expression of p35 and EBI3 mRNA in Dukes C and D stages were significantly higher than that in Dukes A and B stages respectively. The expression of p35 and EBI3 mRNA in lowly- and non-differentiated tumor tissues were significantly higher than those in highly- and moderately-differentiated tumor tissues, respectively. The expression of p35 and EBI3 mRNA in patients with lymph node metastasis were significantly higher than those in no lymph node metastasis patients, respectively. The expression of p35 mRNA has a positive correlation with the expression ofEBI3 mRNA in cancer tissue. Conclusion The expression of p35 and EBI3 mRNA is up-regulated in colorectal cancer tumor tissues and closely associated with clinicopathological features. IL-35 may play an important role in colon cancer tumor microenvironment.%目的：探讨p35和EBI3 mRNA在结直肠癌中的表达并分析其临床意义。方法 RT-PCR检测30例结肠癌组织、30例癌旁组织和20例正常结肠组织中p35和EBI3 mRNA表达情况及与患者临床病理特征的关系。结果结肠癌组织p35和EBI3 mRNA相对表达量均显著高于癌旁组织和正常结肠组织（P均＜0.05）；癌组织中p35和EBI3 mRNA相对表达量均与组织Dukes分期、组织学分型和淋巴结转移相关（P均＜0.05），而与患者的性别和年龄无关（P均＞0.05）。DukesC～D期p35和EBI3 mRNA相对表达量显著高于DukesA～B期，低、未分化结肠癌组织p35和EBI3 mRNA相对表达量显著高于高、中分化组织，淋巴结转移患者癌组织p35和EBI3 mRNA相对表达量显著高于未发生转移患者癌组织。Pearson相关性分析显示癌组织p35和EBI3 mRNA相对表达量呈显著正相关。结论结肠癌患者癌组织p35和EBI3 mRNA高表达与临床病理学特征相关。IL-35在结肠癌患者肿瘤微环境中可能具有重要作用。
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