Expression of P-gp and miRNA-21 in Colorectal Cancer Tissues and the Clinical Significance

摘要

[Objectives]To study the expression of P-gp and miRNA-21 in colorectal cancer tissues and the relationship between the expression of P-gp and clinicopathological features,and to analyze the correlation between the expression of miRNA-21 and P-gp.[Methods]Immunohistochemical SP method was used to detect the expression level of P-gp in the tissues of 40 cases of colorectal cancer and the normal mucosal tissues 5 cm away from the corresponding cancer tissues,and the expression level of miRNA-21 in the paraffin tissues of the 40 cases was detected by RT-qPCR.[Results]The positive expression of P-gp was localized in the cell membrane,and the expression in colorectal cancer tissues was significantly higher than that in the corresponding adjacent tissues(P<0.05);the positive expression of P-gp was closely related to the degree of tumor differentiation and the depth of invasion(P<0.05),and the comparison results of P-gp expression in the differentiation groups of different cancer tissues showed that the expression level of P-gp in the moderately and poorly differentiated groups was significantly higher than that in the highly differentiated group(P<0.05),and the expression level of the moderately and poorly differentiated groups was similar(P>0.05).The expression of miRNA-21 in all cancer tissues was higher than that in the corresponding adjacent tissues(P<0.05),and the expression levels of miRNA-21 in differentiation groups of different cancer tissues were 1.286,1.445 and 1.992 times of those in the corresponding adjacent tissues,respectively;the comparison results of miRNA-21 between different differentiation groups showed that the expression level of miRNA-21 in the poorly differentiated group was significantly higher than that in the highly differentiated group and the moderately differentiated group(P<0.05),while the expression level of miRNA-21 in the highly differentiated group and the moderately differentiated group was similar(P>0.05).Spearman correlation analysis showed that there was a positive correlation on the expression between the two in colorectal cancer tissues(r=0.423,P<0.05).[Conclusions]The expression levels of miRNA-21 and P-gp are closely related to the differentiation degree of clinical colorectal cancer,the lower the differentiation degree,the higher the expression level of the two.