心脏骤停后延迟复苏对大鼠海马病理变化的影响

摘要

目的：探讨心脏骤停后延迟心肺复苏对大鼠海马病理变化的影响。方法成年雄性SD大鼠54只，随机分为假手术组（S组）、即刻复苏组（A组），延迟复苏组（B组），每组按血管穿刺后（S组）或自主循环恢复（ROSC）后（A组、B组）0.5 h断头取海马。A组、B组采用窒息法致大鼠心脏骤停和心肺复苏模型。光学显微镜下观察海马病理变化。结果光镜下，S组海马神经元表现为细胞形态、分布正常，几乎无变性、坏死神经元；与S组比较，A组、B组海马神经元可见明显的细胞水肿、核固缩等病理改变，且B组比A组损伤更为严重。结论延迟心肺复苏不仅降低心脏骤停大鼠ROSC成功率，亦可加重海马组织病理损伤。%Objective To observe the effects of delayed CPR on hippocampal pathological injuries in rats after cardiac arrest. Methods The 54 adult SD rats were randomly divided into 3 groups:sham-operation control group (S group), immediated CPR group (A group),delayed CPR group (B group).Each group was divided on 0.5h after endovascular puncture in S group or after ROSC in A,B group. Asphyxiation cardiac arrest and CPR model was used in A,B group.The pathological changes of hippocampus were observed under light microscope. Results There were serious pathological changes in delayed CPR group. Conclusion The delayed CPR not only reduced the ROSC rate,also aggravated hippocampal pathological damage in rats .