牛奶及乳制品营养丰富,容易消化吸收,人称“白色血液”,是最理想的天然食品.近年来我国乳业发展迅速,但与世界平均水平仍存在巨大的差距,制约我国乳业发展的一个重要原因就是乳糖不耐症.利用β-半乳糖苷酶对牛奶进行水解可生成易被人体吸收的葡萄糖、半乳糖及“双歧因子”低聚半乳糖,不仅能解决乳糖不耐症问题,还能增加牛奶的营养价值.在单因素初步试验的基础上,以低聚半乳糖合成率为响应指标,通过响应分析法对低乳糖水解工艺进行优化.结果表明,当反应温度为45.3℃、加酶量为2.5ml及反应时间为69.7min时,低聚半乳糖得率达到最大值6.15％.通过乳果糖试剂盒测定水解后低乳糖奶中乳果糖含量,平均含量为158.17mg/L.%Nutrient-rich milk and dairy products, easy digestion and absorption, known as "white blood" , is the best natural food. China dairy industry has developed rapidly in recent years, but there is still a huge gap in average level compared to the world. And lactose intolerance is one of the most important reason for restricting the development of China dairy industry. Using β-galactosidase hydrolysis of milk can generate glucose, galactose and the " bifidus factor" galactooligosaccharide, which Not only solve the problem of lactose intolerance, but also increase the nutritional value of milk. On the basis of single factor experiment, the best hydrolysis technique parameter were acquired by response analysis in which the galactooligosaccharide synthesis rate was the main indicator. The results showed that when the reaction temperature was 45. 3 ℃ , enzyme concentration was 2. 5ml and reaction time was 69. 7min, the maximum galactooligosaccharide yield was 6. 15%. The lactulose content in low-lactose milk was 158. 17mg/L determined by lactulose kit.
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