高水解率低乳糖奶的制备及糖类分析

摘要

To determine the optimum conditions for the production of low lactose milk by enzyme method,the hydrolysis process of low lactose milk was optimized by the response surface method,with the hydrolysis rate of lactose as an index.The results were as follows:the ratio of enzyme to substrate ([E]/[S])was 1.50‰,and the hy-drolysis was undertaken at 38.15 ℃ for 3.0 h.Under these condi-tions,the lactose hydrolysis rate of low lactose milk was 92.67%. Moreover,14.02% of the oligosaccharides were detected by HPLC detection,which showedβ-galactosidase could hydrolysis lactose and turn glycosidic.%以乳糖水解率为考察指标,通过响应面法优化低乳糖奶的水解工艺,确定酶法生产低乳糖奶的最佳条件为:酶与底物比例([E]/[S])1.50‰、水解温度38.15 ℃,水解时间3.0 h,该条件下制备的低乳糖奶的乳糖水解率达92.67%,且经高效液相色谱法(HPLC)检测发现有14.02%的低聚糖存在,说明β-半乳糖苷酶既能水解乳糖,又有转糖苷的作用.