魔芋加工下脚料中总生物碱提取技术研究

摘要

In order to increase the additional value, reduce the processing cost and solve the pollution of konjac processing, the alkaloid extraction procedure of total alkaloid from konjac was studied.The alkaloid was extracted using acidity alcohol, with berberine as the control, the alkaloid was determined by acid dye Colorimetry.Four factors influenced the extraction were tested in the single factor experiment including ethanol concentration, solid to liquid ratio,extraction time, extraction temperature.The extracting procedure was optimized by orthogonal experiment.The result showed that the main factors on the extraction efficiency were alcohol concentration and the ratio of sample to alcohol.The optimum extraction conditions were: alcohol concentration 75％, the ratio of sample to alcohol 1: 15, the temperature of water bath 45℃, extraction time 1.5h.%为了提高魔芋资源的利用程度、提高附加值、降低魔芋精粉加工成本、减轻魔芋加工对环境的污染,研究了魔芋加工下脚料中总生物碱的提取工艺技术.本研究以酸性乙醇为提取剂,以盐酸小檗碱为对照,采用酸性染料比色法测定提取液中总生物碱含量,评价提取效果.通过单因素试验考察了乙醇浓度、料液比、提取时间、提取温度四个因素对魔芋加工下脚料中总生物碱提取效果的影响,实验结果表明:适宜乙醇浓度为75%、料液比为1:10、提取温度为50℃、提取时间为2h;通过正交试验确定魔芋总生物碱提取最佳工艺条件,实验结果表明:影响魔芋总生物碱提取效果的主要因素为乙醇浓度和料液比;最佳提取工艺条件为:乙醇浓度75%,料液比1:15,提取温度45℃,提取时间1.5h;本研究的RSD为3.8692%,表明精密度良好.