建立高效液相色谱法测定高粱红色素中芹菜素和槲皮素-7-葡萄糖苷的分析方法.样品经前处理提取溶解,采用C18反相色谱柱分离,用甲醇-1％乙酸溶液(60∶40,v/v)为流动相进行洗脱,检测波长为377nm,流速为1.0 mL/min,以色谱峰保留时间进行定性,外标法进行定量.结果表明,芹菜素和槲皮素-7-葡萄糖苷分别在10.2 ～ 204.0μg/mL和20.4 ～ 408.0 μg/mL范围内线性关系良好,相关系数R2均大于0.999,样品中的加标回收率分别为94.3％ ～ 101.3％和89.4％ ～ 101.6％,相对标准偏差分别为1.8％ ～ 7.9％和2.1％～4.2％(n=3),检出限分别为2μg/mg和4μg/mg,该方法操作简单,精密度好,能满足高粱红色素中芹菜素和槲皮素-7-葡萄糖苷的检测要求.%A new method for the determination of apigenin and quercetin-7-glucoside in sorghum red was developed by HPLC.The sample was extracted with ethylalcohol-HCl (pH3.0).The chromautographic separation was performed on an RP-C18 column,the mobile phase consists of methanol and 1％ acetic acid (60 ∶ 40,v/v) and flow at 1.0 mL/min.The detection wavelength was 377 nm,the retention time was used in qualitative analysis.The external standard method was used in quantitative analysis.The method showed good linearity in the range of 10.2 ～ 204.0 μ g/mL for apigenin,and 20.4 ～ 408.0 μ g/mL for quercetin-7-glucoside with correlation coefficients (R2) all above 0.999.The recoveries were 94.3 ％ ～ 101.3 ％ and 89.4 ％ ～ 101.6 ％ with relative standard deviations (RSDs,n=3) of 1.8％ ～ 7.9％ and 2.1％ ～ 4.2％,respectively.The limits of detection (LODs) were 2 μ g/mg and 4 μ g/mg,respectively.The method was simple with good precision and suitable for the determination of apigenin and quercetin-7-glucoside in sorghum red.
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