维生素E对泥鳅生长性能及消化酶活性的影响

摘要

选取平均体重为（1.58±0.01）g的健康泥鳅540尾，随机分为6组，每组3个重复，每个重复30尾，对照组饲喂基础饲料，试验A、B、C、D、E组饲料中维生素水平分别为24.375、48.75、97.5、195、390 mg/kg，试验期为30 d。结果表明：当维生素E水平为195 mg/kg时，增重率（WG）、特定生长率（SGR）和蛋白质效率（PER）均达到最大值，饲料系数（FC）达到最小值，分别为112.74%、2.51%、241.23%和2.15，均与对照组差异显著（P＜0.01）。维生素E对泥鳅肠道消化酶活性影响显著，195 mg/kg添加组肠道蛋白酶和淀粉酶活性最高而脂肪酶活性最低，分别为14.96、0.206、20.86 U/g prot，均与对照组差异极显著（P＜0.01）。维生素E分别与增重率、特定生长率、蛋白质效率、饲料系数进行回归分析并考虑维生素E对泥鳅消化酶活性的影响，确定泥鳅幼鱼维生素E适宜需要量为156～196.28 mg/kg。%In this experiment,540 healthy Misgurnus anguillicaudatus with (1.58±0.01)g body weight were randomly divided into 6 groups with 3 replicates of 30 each.The control group was fed with basal diet,the experimental groups were fed the feed with the vitamin E levels of 24.375 mg/kg (A),48.75 mg/kg (B),97.5 mg/kg (C),195 mg/kg (D),390 mg/kg (E),respectively.The experiment lasted for 30 d.The result showed that:When vitamin E level was 195mg/kg diet,the fish had the maximal weight gain,special growth rate,protein efficiency ratio,and the feed coefficient achieved the minimals which were 112.74%,2.51%,241.23% and 2.15 respectively,compared with control group,the difference were significant (P<0.01).In addition,vitamin E had significant effect on activities of digestive enzymes of Misgurnus anguillicaudatus(P<0.05).When vitamin E adding amount was 195 mg/kg,experimental fish had the maximal activities of Protease(14.96 U/mg prot)and Amylase(0.206U/mg prot),and had minimal activities of lipase(20.86 U/g prot),respectively.The conic anal-ysis of weight gain,special growth rate,protein efficiency ratio,feed coefficient and the effect of vitamin E level on activi-ties of digestive enzymes of Misgurnus anguillicaudatus indicated that the optimal vitamin E level of Misgurnus anguilli-caudatus was 156~196.28 mg/kg.