婴幼儿食品和乳制品中维生素B12测定的探讨

摘要

Analyze critical points of the method that Determination of vitamin B12 in foods for infants and young children, milk and milk products(GB5413.14-2010). Although the microbiological method is not easy to master, but through summarizing experiences and controlling experimental critical points, the satisfactory results of Method precision and repeatability of GB5413.14-2010 can be obtained. The paper may offer few suggestions for detecting low content of vitamin B12, lower than the instrument limit of detection of vitamin B12 and biological activity of vitamin B12.%针对婴幼儿食品和乳制品中维生素B12测定方法(GB5413.14-2010),分析国标的实验关键点.虽然微生物测定方法不易掌握,但通过实验经验归纳总结和实验关键点控制,GB5413.14-2010方法精密度和重复性都可以得到理想结果.本研究对于质量分数甚微的维生素B12测定、低于仪器最低检出限的维生素测定、具有生物活性的维生素的测定具有一定指导意义.