大白菜游离小孢子培养和植株再生技术研究进展

摘要

综述了影响小孢子培养的主要因素,包括供试材料(基因型、外部环境及生理状况)、小孢子(发育时期和接种浓度)、培养基成分(基本培养基、蔗糖、生长调节剂和活性炭)、培养方式(低温预处理、高温预处理、振荡培养)等;阐述了影响小孢子植株再生的一些因素——胚状体发育阶段、小孢子胚在液体培养基中滞留时间、培养基水分含量、活性炭等；总结了小孢子植株继代与加代技术,并提出了在该领域存在和需要解决的问题,展望了其在研究和生产实践中的应用前景.%Haploid breeding has been greatly developed and isolated microspore culture (IMC) is an efficient method to produce haploid plants of heading Chinese cabbage[Brassica campestris ssp. Pekinensis (Lour. )01sson]. In this paper, the history and recent progresses on IMC of heading Chinese cabbage are reviewed. The major factors affecting the formation of embryo and plantlet regeneration are discussed in detail. The existing problems in this field, the application and production practice are also outlined.