超高效液相色谱测定食醋中的六种防腐剂

摘要

Ultra performance liquid chromatography method is developed for the determination of six kinds of preservatives in vinegar.The analytes are separated by a Waters BEH C18 column (1.7 μm,2.1 mm×50 mm) using 0.01 mol/L of NH4Ac(pH 5.0～5.5)and acetonitrile as mobile phases for gradient elution.A diode array detector is used to detect at 240 nm.The results indicate that six kinds of preservatives are well separated within 8 min.The recoveries at spiked levels range from 93.42％ to 96.28％,the relative standard deviations (RSD) range from 0.75％ to 1.59％.With the advantages of simplicity,sensitivity and repeatability,the method could meet the requirements for determination of preservatives in vinegar.%建立了超高效液相色谱同时测定食醋中6种防腐剂的分析方法.6种化合物经Waters BEHC18 (1.7μm,2.1 mm×50 mm)色谱柱分离,以0.01 mol/L乙酸铵(pH 5.0～5.5)和乙腈为流动相进行梯度洗脱,用二极管阵列检测器在波长240 nm处进行检测.结果表明:6种防腐剂在8 min内可获得良好的分离,加标回收率在93.42％～96.28％之间,相对标准偏差(RSD)在0.75％～1.59％之间.该方法操作简单,灵敏度高,重复性好,可作为检测食醋中防腐剂的方法.