产谷胱甘肽荧光假单胞菌发酵条件的优化

摘要

Methods: During the previous work of the laboratory,a strain of Pseudomonas fluorescens BJYG12 is separated, which has the ability to produce glutathione.On the basis of single factor, the fermentation condition of Pseudomonas fluorescens strain producing glutathione is optimized by the orthogonal test.Results: The fermentation temperature of 28 ℃, original pH of 7.5, inoculum concentration of 5%, culture medium containing yeast extract of 25 g/L,sucrose of 15 g/L, KH2PO4 of 1.5 g/L, MgSO4 of 2.5 g/L.After promoting the fermentation condition,the capacity of strain producing GSH increases by 15.7%.%方法:在实验室前期工作中,分离得到一株可生产谷胱甘肽(GSH)的荧光假单胞菌BJYG12,在单因素的基础上,通过正交试验对该菌株发酵生产GSH的条件进行了优化.结果:发酵温度28 ℃,初始pH 7.5,接种量5%,培养基含酵母膏25 g/L,蔗糖15 g/L,KH2PO4 1.5 g/L,MgSO4 2.5 g/L,优化发酵条件后,菌株产GSH的能力增长了15.7%.