高效液相色谱法测定荷叶中槲皮素的含量

摘要

目的:用高效液相色谱法测定荷叶中槲皮素的含量.方法:采用Phenomenex Luna C18柱(150×4.6mm,5μm),以甲醇:0.05％H3PO4水(60∶40)为流动相,流速1.0mL/min,检测波长360nim,柱温25℃,用外标法定量,测定荷叶中槲皮素的含量.结果与结论:槲皮素的线性范围25μg～200μg,相关系数r=0.9995,回收率99.6％,RSD 1.61％,该方法简便,快速,线性关系良好.%Objective: To determine the content of quercetin in Lotus Leave. Methods: The HPLC method was used to determine the content of quercetin in Lotus Leave. The analysis was carried on Phenomenex Luna C18 column. The mobile phase was MeOH -0.05% H3PQ4(40:60), flow-rate 1.0ml/min, wave-length 360nm and temperature 25℃. Results and conclusion: The method was simple and had good linear relationship.The linear range was 25μg~200μg and gave a correlationship of 0.9995. The recovery was 99.6% and RSD 1.61%. The method was easy in to determineing the content of quercetin in lotus leave.