啤酒废酵母自溶条件的研究

摘要

The key factors of waste brewer's yeast autolysis such as temperature, pH value, addition of NaCl and autolysis time were optimized by single factor tests and orthogonal tests in this article. The results indicated that the optimum conditions were obtained as follows: autolysis temperature 50℃,pH value 5.0, addition of NaCl 3% and autolysis time 24h. Under these autolysis conditions, the content of total sugar would reach 2.27g/L, yield of amino-nitragen was 3.98% and yield of the extract was 54.12% in the supernatant fluid.%通过单因素试验和正交试验对影响啤酒废酵母自溶的温度、pH值、NaCl添加量和自溶时间4个关键因素进行了优化,得到了啤酒废酵母自溶最佳工艺条件:自溶温度50℃,自溶pH值为5.0,NaCl添加量为3％ (w/w),自溶时间24h,自溶上清液中总糖分含量达到2.27g/L,游离氨基酸态氮得率达到3.98％,抽提物得率达到54.12％.