赤水晒醋一株产气微生物的分离鉴定

摘要

为了解赤水晒醋产气原因,采用多种培养基分离和计数产气微生物,并检测其生理生化特征,生长特性以及16S rDNA基因序列分析.实验结果表明,从变质晒醋中分离到一株产气微生物,编号为N9,革兰氏阳性,有芽孢,最适生长温度为45 ℃,最适生长pH值为5.5,最适生长盐度(氯化钠含量)为1％,繁殖能力强.经过16S rDNA基因序列以及系统发育树分析可以鉴定该菌为产碱普罗威登斯菌(Providencia alcalifaciens).%In order to figure out the aerogenesis reason of Chishui sun vinegar,using a variety of culture mediums to isolate and count gas-producing microorganism,and the physiological-biochemical characteristics of strains were detected and 16S rDNA gene sequence were analyzed.The results showed that one gram-positive bacterium with spore,coded as N9,was isolated from the samples of Chishui sun vinegar.The optimum growth temperature of the strain was 45 ℃,pH was 5.5 and the optimum growth salinity (NaCl content) was 1％.The strain had strong reproduction ability.Through the analysis of 16S rDNA gene sequences and phylogenetic tree analysis,the strain N9 was identified as Providencia alcalifaciens.