牛组织蛋白D基因多态性与肉质、胴体性状的关联分析

摘要

4 exons of CTSD gene from 51 Simmental×Luxi catties were amplified and sequenced. 4 SNPs were identified at position 581,599,861 and 1128 bp of CTSD gene(NM 001166521. 1) by sequencing. The effects of 4 mutations on meat quality and carcass traits were analyzed by using general linear model. The results showed that T581C polymorphism significantly associated with marbling trait(P<0. 05). Genotype of BB had better marbling grade than AA and AB genotypes. Thus T581C might be a good marker for marbling traits.%以51头西门塔尔和鲁西黄牛的杂交后代作为试验动物,扩增组织蛋白酶D(cathepsinD,CTSD)基因部分外显子,通过DNA测序方法检测到该基因第5、6、8外显子共4个SNPs(T581C、T599C、A861T、A1128G)的遗传变异.SAS软件采用最小二乘法拟合线性模型,将几个位点的不同基因型与牛肉质、胴体性状进行关联分析.结果表明,位于CTSD基因外显子5的T581C突变与大理石纹性状呈显著相关(P＜0.05),BB基因型显著优于AA和AB基因型,这为T581C作为肉牛大理石纹的分子标记提供了一定的理论依据.