chTLR4及其信号通路在脂多糖致鸡淋巴细胞中的作用

摘要

Toll-like receptor 4 is the transmembrane receptor of lipopolysaccharides(LPS) ;it mediates a variety of injury response induced by LPS.The experiments on chicken lymphocytes induced by LPS with 6,12,24,48 h, to show IL-1β, NF-κB levels,as in-depth study of intracellular TLR4-mediated LPS signaling basis.The results showed that LPS induced cells,IL-1β,NF-κB content increased,both reached the maximum at 24 h,and then began to decreased.The groups in 12,24 h were significantly higher than the control groups (P＜0.05), the groups in 6,48 h groups were not significantly higher than the control groups(P＞0.05).%Toll样受体4(Toll-like receptor 4,TLR4)是脂多糖(lipopolysaccharides,LPS)的跨膜受体,它介导了 LPS诱导机体产生的多种损伤反应.本试验用LPS对鸡淋巴细胞进行6、12、24、48 h诱导,以得到IL-1β、NF-KB水平变化,为深入研究TLR4介导LPS胞内信号传递奠定了基础.结果显示,LPS诱导细胞后,IL-1β、NF-KB含量增多,均在24 h达到最大值,之后开始下降.且在12、24 h与对照组差异显著(P<0.05),在6、48 h与对照组无显著差异(P>0.05).