华蟾毒精对胃腺癌细胞MGC-803作用的研究

摘要

研究华蟾毒精(cinobufagin,CBG)体外对人胃腺癌细胞MGC-803的抑制作用,并初步探讨其抗肿瘤的作用机制.MTT法测定CBG对胃腺癌MGC-803细胞的生长抑制情况;采用光镜观察CBG对人胃腺癌细胞MGC-803形态的影响;流式细胞术检测CBG对MGC-803细胞的抑制作用、细胞周期及细胞早期凋亡的影响.一定浓度范围内CBG对MGC-803细胞的增殖有剂量、时间依赖性抑制作用;光镜下可见细胞形态明显发生变化,细胞变圆,分泌颗粒增多,细胞折光性下降,大部分细胞脱壁;流式细胞仪检测MGC-803细胞,G1期细胞减少,大量细胞停滞在G2/M期,凋亡细胞增多.CBG对MGC-803细胞的抑制作用与诱导其细胞阻滞及凋亡密切相关.%To detect the inhibition of cinobufagin (CBG) on human gastric cancer MGC-803 cells and study its anti-tumor mechanism.The inhibition of cell growth of CBG was measured by MTT assay.Morphological changes were observed by optical microscopy.Apoptosis and cell cycle distribution of MGC-803 cells were determined by flow cytometry.Apoptosis of MGC- 803 cell was detected by Annexin V/PI method.Cinobufagin inhibited the growth of MGC-803 cells.MGC-803 cells treated with cinobufagin showed typical characteristics of apoptosis.The cells became round,cell detachment,membrane blebbing,and cell shrinkage with a condensed cytoplasm.A net increase in the percentage of cells at G2/M phase was encountered,and this phase was maintained throughout the treatment time.Annexin V/PI flow cytometry analysis showed that CBG can cause MGC- 803 cells in early apoptosis.CBG inhibited the growth of gastric cancer MGC-803 cells,induced morphological changes, made cells arrest at G2/M phase and induced apoptosis of MGC-803.The mechanism may be related to the induction of apoptosis and cell cycle arrest.