A STUDY ON THE CYTOTOXIC EFFECT AND EFFICIENT INHIBITION OF CELL CYCLE PROGRESSION OF THE HUMAN BREAST AND LUNG CANCER CELL LINES BY THE DEVELOPED NANO-GELATIN ENCAPSULATED GLUTATHIONE REDUCTASE AND LYCOPENE (IN-VITRO)

摘要

Cancer is the most dreaded disease and is the second leading cause of death worldwide and accounted for 7.6 million deaths (around 13 per cent of all deaths) occurred in low and middle income countries in 2008. Fatalities from cancer worldwide are projected to continue to rise to over 13.1 million in 2030. Based on the current rate of incidence, it is believed that one in every three persons will develop cancer at some time during his life. Nanotechnology is a useful tool for developing ways to eradicate cancer cells without harming healthy, neighbouring cells. Nanotechnology offers a more targeted approach and this could provide significant benefits to cancer patients, hi fact, the use of nanoparticles for drug delivery and targeting is likely one of the most exciting and clinically important applications of cancer nanotechnology. So it was thought of interest to make an attempt to develop nano-gelatin encapsulated GR and Lycopene from the commonly consumed food, tomato in combating cancer by sustaining their stability, as nanomedicine in future would play a crucial role in treatment of human diseases and also in enhancement of normal human physiology. From the results obtained in the study, it is evident that there was a feasibility of synthesizing nano-gelatin encapsulated GR and Lycopene and these compounds were found to be stable at 4°C for a period of more than 90 days and also the nano-size of 100 nm of these compounds was evident through characterization using UV-Visible spectroscopy, SEM and FTIR analysis. It was exciting to note that both Glutathione Reductase and Lycopene in nano-gelatin encapsulated forms proved to have more cytotoxicity and are also capable of efficiently inhibiting the cell cycle progression at various phases of the cell cycle in human breast and lung cancer cells when compared to the extracted forms. Apart fi-om this both the encapsulated compounds were found to be non-toxic to normal vero (healthy) cells. Abbreviation - GR - Glutathione Reductase, SEM - Scanning electron microscopy, FTIR - Fourier transform infrared spectroscopy.