几株鸡新城疫病毒的分离鉴定及其基因型分析

摘要

Six strains of virus isolated from several areas of poultry occurred Newcastle disease, through HA, HI, neutralization test, and molecular biology experiments were identified as Newcastle disease virus. According to F gene sequences published in GenBank, a pair of specific primers was designed and synthesized, length of approximately 610 bp was amplified, a-lignment and analysis of nucleotide and amino acid sequences were conducted. The results showed that there were 3 lines with virulent Newcastle disease virus strain characteristics, also had the Newcastle Ⅵ genotype features. According to MDT, ICPI and IVPI index determination result, we finally determined that the 3 strains were virulent strains of NDV and belonged to Ⅵ genotype. The homology of nucleic acid and amino acid sequence between remaining 3 strains of virus and La Sota were both 99%.%从几个发生禽病的地区分离到6株病毒,经过血凝试验、血凝抑制试验、中和试验、分子生物学试验等确定为鸡新城疫病毒；根据GenBank公布的新城疫F基因强弱毒株相关基因序列设计合成1对特异性引物,扩增出的F基因片段长度约为610 bp,测序后进行序列比对,并分析其核苷酸序列和氨基酸序列,结果显示,有3株具有新城疫强毒株特性,同时还具备新城疫Ⅶ基因型特征,另外根据平均死亡时间(MDT)、脑内致病指数(ICPI)和静脉致病指数(IVPI)指标测定结果,最终判定这3株是新城疫强毒株且属于基因Ⅶ型,其余3株病毒分离株与La Sota的核酸序列同源性与氨基酸序列同源性均为99％.