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DEVICE FOR GENOTYPIC ANALYSIS AND METHOD FOR GENOTYPIC ANALYSIS

机译:基因型分析装置和基因型分析方法

摘要

A technique for performing spectral calibration simultaneously with electrophoresis of an actual sample to be analyzed, without performing electrophoresis using a special matrix standard which is time-consuming and costly, is provided. The device for genotypic analysis is characterized by obtaining reference fluorescence spectra using a size standard and an allelic ladder, which provide information concerning known DNA fragments used for electrophoresis of an actual sample, and is characterized by performing spectral calibration for a capillary in which the allelic ladder is not used by detecting a shift amount of the fluorescence spectra of the size standard and shifting the reference fluorescence spectra using the shift amount to determine fluorescence spectra.
机译:本发明提供了一种技术,该技术用于在进行要分析的实际样品的电泳的同时进行光谱校准,而无需使用费时且昂贵的特殊矩阵标准进行电泳。基因型分析装置的特征在于,使用大小标准和等位基因梯获得参考荧光光谱,从而提供有关用于实际样品电泳的已知DNA片段的信息,并且其特征在于,对其中等位基因的毛细管进行光谱校准通过检测尺寸标准的荧光光谱的偏移量并使用该偏移量偏移参考荧光光谱以确定荧光光谱,不使用梯形图。

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