Analysis on Genotypic Differentiation of Phytophthora infestans by Using Random Amplified Polymorphic DNA (RAPD)

摘要

A total of 104 isolates including two Korean isolates and three Japanese isolates of Phytophthora infestans collected from Heilongjiang and Jilin Provinces from 2006 to 2008 were used to determine their mating types,metalaxyl resistance,and RAPD genotypes.All the isolates of P.infestans collected from Heilongjiang and Jilin Provinces belonged to A 1 mating type,and no A 2 mating type was detected.Frequencies of metalaxyl resistant isolates were 94.4%,47.8% and 75.0% in 2006,2007 and 2008,respectively.According to RAPD analysis by using OPD-20 primer,104 isolates including two Korean isolates and three Japanese isolates were separated into 10 RAPD groups at 90.8% similarity level.A total of 10 RAPD groups were not correlated to groups defined by mating type,locations,and metalaxyl resistance.