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东北农业大学学报：英文版

东北农业大学学报：英文版

《东北农业大学学报（英文版）》创刊于1994年，为东北农业大学主办的农业自然科学综合性学术期刊，是目前我国农业高校学报中唯一的一本英文版期刊，也是我国农业行业里为数极少的英文期刊中创刊最早、发展较快、并具有一定影响力的学术期刊。主要刊登农学、园艺、植保、资环、动物科学、动物医学、农业工程、农业水利、生物工程、食品科学、基础科学等方面的科研学术论文，及国内外有关学科符合本刊宗旨及办刊内容的论文。.
  • 发文量：1177
  • 被引量：272
  • H指数：4
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  • 国内刊号/CN：23-1392/S
    国际刊号/ISSN：1006-8104
  • 发行周期：季刊
    邮发代号：14-250
  • 主管单位：东北农业大学
    主办单位：东北农业大学
东北农业大学学报：英文版-联系信息
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  • 摘要：In order to observe the role of calcium ion in apoptosis of MD cancer cells induced by arsenic trioxide,inhibition percentage was detected by MTT assay;morphology changes were examined by fluorescence microscope;apoptosis was examined by DNA Ladder;Ca2+ i was investigated by spectrofluorimeter in vitro on MDCC-MSB1 cells.The results showed that As2O3 inhibited the proliferation of MDCC-MSB1 cells in concentration dependent manner(P<0.05 or P<0.01);typical apoptosis character was observed by fluorescence microscope;DNA Ladder was observed;the Ca2+ i was elevated significantly after the treatment of As2O3(P<0.05 or P<0.01) and showed a dose-dependent manner.It is concluded that the calcium may play an important role in apoptosis of MD cancer cells induced by arsenic trioxide.
  • 摘要：Germplasm effect reflects the quantitative relation between production ability of germplasm elements and yield(quality) of a certain crop,which can be shown by mathematic function,namely,germplasm effect function.Germplasm effect of a crop variety is an aggregation of many effective factors,and is restrained by different effective factors;constant increase of any one effect of germplasm elements would lead to law of effect decline,therefore,possible modes of transgenic crops effect function were deduced according to the law of effect decline.The possible modes of single transgenic germplasm effect function and multi-transgenic germplasm effect regression equation were discussed,and the characteristics of germplasm effect regression equation were analyzed in this paper.
  • 摘要：The gene regulatory network was reconstructed according to time-series microarray data getting from hybridization at different time between gene chips to analyze coordination and restriction between genes.An algorithm for controlling the gene expression regulatory network of the whole cell was designed using Bayesian network which provides an effective aided analysis for gene regulatory network.
  • 摘要：The optimum medium for dormant axillary buds culture of blue honeysuckle was screened according to the growth rate and elongation rate by inoculating the buds on culture medium with various 6-BA and iron-salt concentration.About 35 days,the stretched stem buds were divided into strong root system after inoculated on 1/2 MS+1.0 mg · L-1 IBA rooting medium.Amount of qualified tissue-cultured young plants could be obtained by the stretched stem buds reproduction.
  • 摘要：sIgA in bovine colostrum was purified by ultrafiltration and enzymolysis methods in this experiment,and the prepared substance was detected by Western Blot.The purity and yield were up to 73.6% and 65.2%,respectively.This convenient technique offers helpful exploration for production of bovine colostrum sIgA.
  • 摘要：A total of 12 mature healthy Holstein dairy cows of the nearly body weight(580 ± 30) kg,milk yield(22.5 ± 2.8) kg in the early stages of lactation were selected in this experiment.The cows were randomly divided into 2 groups,every group had 6 cows,every group had 6 repeats,and every repeat had 1 cow.Added 20 g protected methionine in earlier lactating cow food every day.The results showed that protected methionine increased milk yield by 10.83%,testing group milk yield was significantly different than that of control(P0.05);dry matter of milk had the tendency of decrease,but had insignificant difference(P>0.05).
  • 摘要：The paper introduced CAN(Controller Area Network) bus and CAN2.0 standard,the design of hardware frame-work multi-point communication based on CAN bus,and the software design method.At the same time,the system normal communication function and stability were shown in the experiment.
  • 摘要：This article introduced the new methods on the research of the wild flowers and plants idioplasmic resources,elaborated the introduction and domestication and exploitation of wild flowers and plants idioplasmatic resources and the sustainable development of flowers and plants industry in China,and put forward some proposals on the existing question and the prospects for the development.
  • 摘要：In this paper,the backdrop and significance of the research of germ-remaining rice whitener firstly were expatiated.Then,the study progress in China and overseas was summarized.Lastly,the research prospect of this kind of whitener in China was analyzed.It is hoped that it can coach the design of germ-remaining rice whitener.
  • 摘要：As a new kind of analytical instrument,the principles,types and characters of biosensors were discussed in this paper.A biosensor is usually composed of a recognition element of biological origin and a physicochemical transducer.The biological element is capable of sensing the presence,activity or concentration of a chemical analyse in solution.The sensing takes place either as a binding event or a biocatalytical event.These interactions produce a measurable change in a solution property,in which the transducer is converted into a quantifiable electrical signal.The principles,types and applications of biosensors in environmental inspection,food production,clinical medicine and military defense were reviewed,and the trends in research were predicated.Furthermore,an attempt had been made to describe the future development directions and prospects.
  • 摘要：This paper made an analysis on the structure and function of MapX.Based on the relative GIS software and development theories,general design of GIS foundation platform was put forward and some key implementations for part of the function was provided.
  • 摘要：MicroRNAs (miRNAs) are one kind of small RNA in all eukaryote. MicroRNAs can regulate gene expression of eukaryote; they widely participate in every physiological process. They can block mRNA expression or cleave mRNA by complement to target mRNA. Scholars estimate miRNA genes occuping about 1% of genome, but they can regulate 10%-30% genes of whole genome. The genes are regulated by miRNA including signal proteins, enzymes, transcription factors, and so on. In the field of plant research, the start of miRNA research is later, but it is proved that plant miRNAs are important to every plant physiological process. Now miRNA has become the hotspot of plant molecular biology research. This paper introduced the biology function, action mechanism, researching method and recently development of microRNAs, also focused on advances in plant microRNAs. This paper has important reference value for plant stress tolerance miRNA research.
  • 摘要：Attaching more importance to the problem of animal excrement pollution is the premise of realizing the sustainable development of animal husbandry in Heilongjiang Province. With the constant expansion of the scale of breeding industry, the amount of animal excrement will increase greatly, corresponding to the increasing pressure on water and land. Therefore, for the sake of promoting the coordinated development of animal husbandry and the environment, this paper analyzed the status of animal excrement pollution in Heilongjiang Province. On this base, the paper suggested that we must control animal excrement pollution proceeding from reality by using administrative means, legal means, economic means and technical means.
  • 摘要：The extensively built long-distance water transmission pipelines have become the main water sources for urban areas. To ensure the reliability and safety of the water supply, from the viewpoint of overall management, it would be necessary to establish a system of information management for the pipeline. The monitoring, calculating and analyzing functions of the system serve to give controlling instructions and safe operating rules to the automatic equipment and technician, making sure the resistance coefficient distribution along the pipeline is reasonable; the hydraulic state transition is smooth when operating conditions change or water supply accidents occur, avoiding the damage of water hammer. This paper covered the composition structures of the information management system of long-distance water transmission pipelines and the functions of the subsystems, and finally elaborated on the approaches and steps of building a mathematics model for the analysis of dynamic hydraulic status.
  • 摘要：Fusarium wilt caused by Fusarium oxysporum f.sp. cucumerinum (Owen) is one of the most devastating diseases in cucumber production worldwide. Recent progresses in the mechanism of resistance to Fusarium wilt in cucumber were reviewed in this paper, including pathogenic mechanism of Fusarium oxysporum, the resistance mechanism of cucumber, the heredity of resistance, and the location of resistance genes. Following works should be the location and cloning of resistance genes with molecular biologic methods.
  • 摘要：The p53 and CD44 expression of 10 cases in canine breast tumor were examined utilizing immunohistochemical assay with rabbit anti-mouse polyclonal antibodies against p53 or CD44, respectively. The p53 expression was significantly higher in malignant than in benign breast tumor. The expression of CD44 was not significantly different in malignant breast cancer and benign breast tumor. This suggests that p53 can be used as an indicator for animal prognosis.
  • 摘要：Under the premise of unchanging the collective ownership and the mode of family business, China should choose land shares pack system as the goal pattern of farmland system innovation. It is hoped that the pattern is accepted and supported by both sides of the government and the farmers, and then it constructs the land reasonable circulation and the effective centralism mechanism, thus realizing smooth transition from the traditional agriculture to the modern agriculture.
  • 摘要：The small envelope protein (E) gene of avian infectious bronchitis virus (IBV) M41 strain was cloned, and then it was subcloned into prokaryotic expressing vector pGEX-6P-1. The recombinant plasmid was transformed into E.coli. BL21 and induced by IPTG. SDS-PAGE result showed that when objective protein fused with GST (about 20 ku), the relative molecular mass of fusion protein was 38 ku. It indicated that objective protein was about 12.4 ku. The result showed that E protein was expressed successfully, it was useful to the subsequent E protein research.
  • 摘要：Hyaluronic acid (HA) had been prepared from pigskin residues with neutral proteinase. The preparing results and conditions were studied. After extracting and purification, HA was detected through ultraviolet spectra and infraction spectrum, and its content and purity were tested by carbazole and Elason-Morgan, respectively. This results indicated that significant quantities of HA could be prepared in fresh pigskin with biologic enzyme, and the pure HA was cosmetic grade and food grade.
  • 摘要：In order to compensate for the deficiency of Sine Pulse Width Modulation(SPWM), on the base of analyzing the principle of space vector pulse width modulation and being compared with SPWM, the method of solving working time of adjacent vector and the method of generate space voltage vector were introduced. The experiment to the inverter which consists of IGBT proves that SVPWM control algorithm can reduce harmonic effectively, it is beneficial to enhancing the utilization rate of voltage source inverter direct current power supply.
  • 摘要：The ISSR method was developed to analyze the genetic diversity of 20 wild Wusuli raccoon dog from Nenjiang district. The results showed that there were significant genetic diversity and high polymorphism among individuals of the wild Wusuli raccoon dog. A total of 41 DNA bands were amplified by 9 primers, and 37 of them were polymorphism, the proportion of polymorphism was 90.24%, 2-8 polymorphism bands could be amplified by each primer, and 4.56 on average, the length of the product was 200-2 000 bp.
  • 摘要：This paper used fuzzy math principles, analyzed different aspects of the work of the current rural endowment insurance and formed the indices to assess the work level of the rural endowment insurance. After selecting the indices of the work level scientificly, constructed the index system to evaluate the work level of the rural endowment insurance and the index system was expressed in the form of math equation. In this way, an objective standard used to evaluate the work level of rural endowment insurance was establised.
  • 摘要：Most important agricultural traits of crops are controlled by many genes. These traits have complicated genetic basis and are difficult for genetic analysis. Due to application of molecular marker techniques in the last two decades, genetic and molecular dissection of quantitative traits has become possible. In this paper, recent progress on mapping of quantitative trait loci in crops was reviewed.
  • 摘要：This study investigated the growth situation of Listeria monocytogenes on chilled pork and the effect of herbal preservatives on this pathogen. The inhibitions of herbal preservatives were identified. The minimum inhibitory concentrations (MIC) of cinnamon and clove were all 0.79 mg·mL-1, while the rosemary was 1.58 mg·mL-1. And the composite herbal preservatives were got through orthogonal experiment. The optimum proportion was as following on agar medium: 1.16 mg·mL-1 cinnamon+2.38 mg·mL-1 rosemary+3.17 mg·mL-1 clove (herb combination number 5), while on chilled pork, the strong inhibition of L. monocytogenes was showed, which demonstrated that the surface application of herb combination resulted in an effective delay of L. monocytogenes growth.
  • 摘要：The effect of moisture conditioned for brown rice (moisture content of 1.45%) on milling characteristic was studied in this paper. Milling experiments were conducted for the brown rice, which were conditioned under different amount of humidification and duration. Software SAS was used to analyze the effect of amount of humidification and duration on the head rice yield and energy consumption of milled brown rice. The reasonable parameters including amount of humidification of 1.45% and duration of 60-90 min were obtained, which decreased energy consumption by 30% and increased head rice yield by 10%. Therefore, the method of moisture conditioned can improve milling quality of stored paddy due to the hardness and crisp of paddy reduced.
  • 摘要：In this paper, the application of bluetooth in vehicle hands free system was described, then the hardware and software structure of system had been designed. Finally, selection about electron parts and chip of electronic equipments was presented in detail.
  • 摘要：By the observation of chloroplast ultrastructure in different period of bract colors of Euphorbia pulcherrima, the paper studied the change of chloroplast ultrastructural in the transition process of bract colors, identified the relationship between E.pulcherrima color change and the chloroplast ultrastructure to provide theorical bases for the cultivation management and further study of E. pulcherrima. Ultrastructural study showed that in the process of change from green to red, the chloroplast of bracts disintegrated gradually, lamellar structure was destroyed gradually, and the content of chloroplasts in mesophyll cells was also reduced gradually. When bracts color resumed to turn green gradually, the content of chloroplasts in mesophyll cells was also increased gradually.
  • 摘要：The effects of spices(cinnamon,rosemary,clove)extracts and chitosan on microbiological growth,drip loss,color,and lipid oxidation of fresh chilled meat stored for 28 days at 4℃were investigated.There were four treatments:control,coated with spices(cinnamon 1.5 g?L-1+rosemary 1.5 g?L-1+clove 1.0 g?L-1),coated with 0.5%chitosan,coated with spices and chitosan.Chitosan coating resulted in significant inhibition of microbial growth(P<0.05),while the lowest microbial counts were obtained in the samples containing both chitosan and spices,indicating a possible synergistic effect.Chitosan and its combinations with spices also showed the most intense antioxidative effect when compared to the controls(P<0.05).Meanwhile,chitosan coating could decrease water loss and keep better color of chilled meat.The combined spice extracts and chitosan coating could effectively extend the shelf life of chilled meat.
  • 摘要：Specificity of the plant innate immune system is often conferred by resistance(R)proteins.Most plant disease resistance (R)proteins contain a series of leucine-rich repeats(LRRs),a nucleotide-binding site(NBS),and a putative amino-terminal signaling domain.They are termed NBS-LRR proteins.The LRRs are mainly involved in recognition,and the amino-terminal domain determines signaling specificity,whereas the NBS domain presumably functions as a molecular switch.During the past years,the most important discoveries are the role of partners in NBS-LRR gene mediated defenses,mounting support for the so-called"guard hypothesis"of R gene function,and providing evidence for intramolecular interactions and intermolecular interactions within NBS- LRR proteins as a mode of signaling regulation.The outcome of these interactions determines whether a plant activates its defense responses.
  • 摘要：There has been a rapid development in the construction of wetland parks in China in recent years.This paper discussed the progress in the research of Chinese wetland parks that covered the aspects of wetland resources,landscape and environment.The function of wetland parks,the evaluation of landscape and the exploiting of resources;the landscape concept and landscape planning and design;the plant environment,the water environment and the recreation environment were all reviewed.The research of Chinese wetland parks started later but developed rapidly,having remarkable achievements in practice and functional studies,and there are still some shortcomings in the researches to be improved.At last,the key points which should be researched urgently in the future were discussed.

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