奶牛乳腺上皮细胞核蛋白质组双向电泳方法的建立

摘要

The resolution of 2-DE map is highly altered due to different experimental conditions, a 2-DE map with high resolution can be obtained via the optimization of key experimental conditions for a definite proteome sample. To establish an effective separation system of 2-DE for the proteome of bovine mammary epithelial cell nucleus, 2-DE method was researched on the standard experimental condition with an optimization for each important experimental parameter. The numbers of protein spots, resolution and contaminating strands in 2-DE maps were determined. A 2-DE separation system for proteome of bovine mammary epithelial cell nucleus proteins was successfully established.%蛋白质样品的双向电泳分离效果受各种试验条件的影响较大,针对不同来源的蛋白样品进行试验条件的优化可获得具有较高分辨率的双向电泳图谱.试验拟建立优化的奶牛乳腺上皮细胞核蛋白质双向电泳分离体系,按标准条件对蛋白质进行双向电泳分离,并对关键因素进行优化,采集电泳图谱并分析双向电泳图谱中蛋白斑点的数量、图像分辨率及背景条纹的变化.通过对试验条件的筛选和优化,成功建立了具有较高分辨率的奶牛乳腺上皮细胞核蛋白质组双向电泳分离体系.