Bidirectional electrophoresis method and bidirectional electrophoresis apparatus

摘要

PROBLEM TO BE SOLVED: To provide a bidirectional electrophoresis device, which can be used from a reverse direction of a separation passage, when adsorption of protein, or the like, to the inner wall of the separation passage or an injection-side trouble occurs, and reduces analysis cost.;SOLUTION: The device includes a holding substrate 21, having an optical window 22 extending from the upper surface to the lower surface thereof; transparent microchips 15, 17 mounted on the holding substrate 21 and including a separation passage 16 for electrophoresis and sample reservoirs 13a and 13b, connected to both ends of the separation passage 16; electrodes 11a and 11b for applying a high voltage necessary for electrophoresis to the separation passage 16; and an optical measuring system 5 for measuring electrophoresis in the separation passage 16 via the optical window 22. The direction of the microchips 15 and 17 is set to a reverse direction relative to the holding substrate 21 together with the electrodes 11a and 11b, thereby electrophoresis is made to be carried out in the reverse direction in the separation passage 16.;COPYRIGHT: (C)2010,JPO&INPIT