本试验旨在了解北京地区鱼源病原菌对氟喹诺酮类药物的耐药现状,提供氟喹诺酮类药物在防制鱼类疾病上合理规范使用的依据.从北京地区具有典型症状的病鱼脏器或病灶分离出16株病原菌,采用纸片扩散法检测病原菌对7种氟喹诺酮类药物的耐药表型,运用PCR法分析病原菌的喹诺酮类耐药基因gyrA、qnrA和qnrS的携带情况.结果表明,鱼源病原菌对氟喹诺酮的耐药基因阳性率显著高于耐药表型检出率,可能耐药基因检测比耐药表型检测更能推测病原菌的耐药现状.50％的病原菌至少耐受1种氟喹诺酮类药物,87.5％的病原菌至少携带1种喹诺酮耐药基因,显示北京地区鱼源病原菌对氟喹诺酮类药物已在基因水平呈现出严重的耐药,有必要严格控制此类药物在鱼类养殖上的应用.%In order to survey the status quo of fish pathogens resistant to fluoroquinolones sourced from Beijing area, and provide the basis to regulate the fluoroquinolones used in fish farming,there were 16 pathogens isolated from diseased fish organs or focus with typical symtom in Beijing, and then the resistance phenotype of fish pathogens on seven kinds of fluoroquinolones were assayed by Kirby-Bauer disk diffusion method according to CLSI and the status of which carried gyrA,qnrA and qnrS genes were detected by PCR method. The data results showed that, on one hand, the rate of fish pathogens detected quino-lones resistance gene-positive was higher than that of resistance phenotype,and the reason might be that the resistance genes detection were more exact than resistance phenotype tested. On the other hand, half of the fish pathogens resistant to a kind of fluoroquinolones at least, while 87. 5% of the pathogens carried a quinolones resistance gene at any rate. Thereby.it was serious that the fish pathogens sourced from Beijing resistant to fluoroquinolones at genetic level, and it was necessary to strictly control these drugs used in fish farming.
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