暴露于环境烟气中的大鼠尿样代谢物分析

摘要

4-(甲基亚硝胺基)-1-(3-吡啶基)-1-丁醇(NNAL)是烟草特有亚硝胺4-(甲基亚硝胺基)-1-(3-吡啶)-1-丁酮(NNK)在生物体内的一种代谢标记物,分析暴露于烟气中的生物体内NNAL的含量是研究卷烟烟气对生物体健康影响的有效手段.基于人体的个体差异性很大,本文以饲养的大鼠为研究对象,采用LC-MS/MS测定暴露于环境烟气中的大鼠尿样4-(甲基亚硝胺基)-1-(3-吡啶基)-1-丁醇(NNAL)代谢标记物的含量.2mL大鼠尿样通过分子印迹柱(MIP)分离富集,电喷雾离子化质谱检测.分析了暴露于环境烟气中1个月和2个月后大鼠尿样中总NNAL.方法的检出限为1.6 pg/mL尿样,精密度为7.8%～11.2%,平均回收率为87.1%.结果表明暴露于环境烟气中1个月和2个月的大鼠尿样NNAL含量没有区别(p＞0.05),大鼠尿样中平均NNAL浓度为20.9±14.6 pg/mg肌酐,浓度范围为6.3～50.2 pg/mg肌酐.%4-methylnitrosamino)-l-(3-pyridyl)-1-butanol (NNAL), a metabolite of the tobacco-specific lung carcinogen 4-( methylnitrosamino)-1-(3-pyridyl)-l-butanone (NNK), was analyzed in the rat urine to assess the exposure of NNK in the tobacco smoke. The rats were exposed to environmental tobacco smoke. Urine samples were collected every month, all in two months.Quantitative analysis of was carried out by combined liquid chromatography-tandem mass spectrometry using a simple cleanup based on a molecularly imprinted polymer (MIP) column. 2 mL of urine was hydrolyzed by β-glucuronidase and the total NNAL was enriched on the MIP column following the determination of LC-ESI-MS/MS. The detection limit of method is 1.6 pg/mL. The precision is from 7. 8 ％ to 11.2 ％ and the mean recovery is 87. 1％. The total urinary NNAL levels for the first month and the second month were compared. The results show that the presence of NNAL in the rat urine of the exposed subjects. There no significant difference in the total NNAL levels between the first month and the second month (p ＞0. 05). The mean level of NNAL was 20. 9 ± 14. 6 pg/mg creatinine; range, 6. 3-50. 2 pg/mg creatinine.