临床诊室浓度的艾烟暴露12周对Wistar雄性大鼠尿常规的影响

摘要

Objective:To investigate the effects of the 12-week moxa smoke intervention on urinary parameters in Wistar rats . Methods:An automatic dynamic exposure device was applied for whole-body mode exposure .The moxa smoke concentration was represented by shading rate ( SR:x%) .Forty-eight male Wister rats were randomly divided into 4 groups ( n=12/group ):three moxa smoke groups according smoke concentrations (L 0.4%,M 2.0% and H 15%) and one control group (C).Three moxa smoke groups were respectively 1,3,and 9 times the clinical concentration .The exposure lasted for 12 weeks (200 min/d and 5 d/week).After the exposure,urine samples were tested by uric dry chemical analysis method .Results:Compared with C group,no statistically differences in the color,SG,PRO,GLU,BLD,URO,BIL were found in smoke-exposed groups (P>0.05).Conclu-sion:The 12-week moxa smoke exposure induced no smoke-related toxicity on urinary parameters in male Wistar rats .%目的:观察临床诊室浓度的艾烟暴露12周对Wistar雄性大鼠尿常规影响.方法:应用全自动动式染毒柜,艾烟的浓度以遮光率SR表示.将Wistar雄性大鼠48只随机分成4个艾烟浓度梯度组(n=12):对照组(C,SR 0%)、低浓度组(L,SR 0.4%)、中浓度组(M,SR 2%)、高浓度组(H SR 15%),分别为无艾烟组,1,3,9倍于临床浓度艾烟组.暴露200 min/d,5 d/周,连续暴露12周.应用尿干化学分析法检测大鼠尿常规等指标.结果:艾烟暴露各组尿的颜色、SG、PRO、GLU、BLD、URO和BIL检查等均未见显著改变(P>0.05).结论:临床诊室浓度的艾烟暴露未对雄性大鼠尿常规指标产生毒性影响.