耐辐射奇球菌对Cu2+和Cr6+吸附行为研究

摘要

In this article,Deinococcus radiodurans was used as biosorbent to remove Cu2+and Cr6+from aqueous solution.The key influencing factors including adsorption time,initial ion concentration and concentration of fluid were observed to investigate the ad-sorption ability of Deinococcus radiodurans.We also investigate the optimum adsorption conditions and identify the competitive ad-sorption behavior.UV spectrophotometer was used to analyze the adsorption efficiency in different conditions.Fourier Transform in-frared spectroscopy(FTIR)and scanning electron microscopy(SEM)were used to analyze the structure and morphology changes of bacteria before and after adsorption.The results showed that the optimum adsorption time of Cu2+and Cr6+was 80min.The optimum initial concentrations of Cu2+and Cr6+were 0.08mol·L-1and 0.138mol·L-1,respectively.With the increased concentration of Deinococcus radiodurans,the adsorptive capacity of Cu2+and Cr6+were increased simultaneously.Competition research showed that Cu2+was adsorbed more strongly on the biomass than Cr6+.The adsorption behavior of Cu2+and Cr6+was demonstrated by Fourier Transform infrared spectroscopy and scanning electron microscopy. Deinococcus radiodurans showed potential application in wastewater treatment because of its strong survival ability,non-pathogenic and environment friendly.%本文以耐辐射奇球菌作为生物吸附剂去除溶液中的Cu2+和Cr6+,通过考察吸附时间、初始离子浓度、菌液浓度等影响吸附效果的关键因素,研究了耐辐射奇球菌对Cu2+、Cr6+的吸附作用,找出最佳吸附条件,并研究了Cu2+、Cr6+共存条件下的竞争吸附行为.采用紫外分光光度计分析耐辐射奇球菌在不同条件下对Cu2+和Cr6+的吸附作用;傅立叶红外光谱(FTIR)和扫描电镜(SEM)分析细菌吸附前后的结构和形貌变化.结果表明:耐辐射奇球菌对 Cu2+和 Cr6+的最佳吸附时间均为80min.对 Cu2+、Cr6+吸附的最佳初始浓度分别为0.08mol·L-1、0.138mol·L-1,且随着菌液浓度的增大,耐辐射奇球菌对 Cu2+、Cr6+的吸附量随之增加.当Cu2+、Cr6+共存条件下耐辐射奇球菌对Cu2+吸附量较Cr6+更大.红外光谱仪和扫描电镜结果阐释了耐辐射奇球菌对Cu2+和Cr6+的吸附行为.耐辐射奇球菌以其超强生存能力,非致病性,环境友好等优势,在重金属废水处理方面将会有很好的应用前景.