( PAA-b-F127-b-PAA)-Blg复合物的合成及乳化性能

摘要

采用原子转移自由基聚合(ATRP)方法合成了PtBA-b-F127-b-PtBA(PtBA为聚丙烯酸叔丁酯),经三氟乙酸水解得到PAA-b-F127-b-PAA(PAA为聚丙烯酸).进一步通过碳二亚胺的水溶性衍生物1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC·HCl)在水溶液中成功地耦合β-乳球蛋白(Blg)和PAA-b-F127-b-PAA.蛋白的三级构象没有受到反应条件的影响.与纯Blg相比,(PAA-b-F127-b-PAA)-Blg复合物的乳化性能大大提高.%Poly (acrylic acid) -b-F127-b-poly( acrylic acid) (PAA-b-F127-b-PAA) was synthesized through atom transfer radical polymerization (ATRP) in combination with the subsequent hydrolysis of iert-butyl groups using excess trifluoroacetic acid(TFA). The (PAA-b-F127-b-PAA)-j8-lactoglobulin conjugate was successfully syn thesized using water soluble l-ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride( EDC ? HC1) , a carbodiimide derivative, as the conjugation agent. Fluorescence spectroscopy indicated that the ternary struc ture of protein was maintained even conjugation to PAA-b-F127-b-PAA. The conjugate exhibited significantly better emulsifying properties than that of pure β-lactoglubulin.