HPLC法同时测定乳酸菌饮料中2种防腐剂和3种甜味剂

摘要

建立了一种高效液相色谱法同时测定乳酸菌饮料中2种防腐剂(苯甲酸、山梨酸)、3种甜味剂(乙酰磺氨酸钾、天门冬酰苯丙氨酸甲酯、糖精钠)的方法.样品经12％乙酸锌溶液和12％亚铁氰化钾溶液处理后,用C18色谱柱分离,以甲醇和0.02 mol/L乙酸铵溶液为流动相梯度淋洗,紫外检测波长为230,205 nm,可在30 min内将5种组分完全分离,测定结果的相对标准偏差为1.1％～4.3％(n=5),加标回收率为90％～102％.%A rapid and selective HPLC method was developed for the simultaneous analysis of two preservatives and three sweeteners including benzoic acid, sorbic acid, acesulfame potassium (acesulfame-K), L-aspartyl-L-phenylalanine methyl ester (aspartame), saccharin sodium in lactic acid bacteria beverage. The preservatives and sweeteners in samples were extracted with 12% zinc acetate solution and 12% potassium ferrocyanide solution, separated on a Cl8 column using a mobile phase made up of methanol - 0.02 mol/L ammonium acetate and ultraviolet detection wavelength was 230,265 nm. All the five additives were separated completely in 30 minutes. The recovery was in the range 90% - 102%, and the RSD of determination results was 1.1%-4.3%(n=5).