用不同方法评价了大麦糟多酚的抗氧化活性，如测定大麦糟多酚的还原能力、羟基自由基、超氧阴离子自由基以及DPPH自由基的清除能力。试验结果表明，大麦糟多酚清除羟基自由基、超氧阴离子自由基和DPPH自由基的能力较强，大麦糟多酚精提取液的半抑制浓度（IC50）分别为4．132、89．504、15．304μg／ml ，而粗提取液的半抑制浓度分别为87．408、113．795、26．873μg／m l；在测定大麦糟多酚的还原能力试验中，当吸光值为0．6时，儿茶素、V C、大麦糟多酚精提取液和粗提取液的质量浓度分别为48．8、54．8、44．1和72．9μg／m l ，大麦糟多酚精提取液体外抗氧化能力明显优于儿茶素、VC和粗提取液。%The antioxidant activity of barley spent gains was investigated with different methods .The reductive ability and the scavenging of hydroxy free radical ,superoxide anion free radical ,DPPH free radical of barley spent gains polyphenols were used to evaluate the antioxidant activity .Results demonstrated that polyphenol from barley spent grains showed high scaven‐ging activity of hydroxyl radical ,superoxide anion radical and DPPH radical ,the concentrations for 50% inhibition of essence extract were 4 .132 ,89 .504 ,15 .304μg/ml ,respectively ,the concentrations for 50% inhibition of crude extract were 87 .408 , 113 .795 ,26 .873 μg/ml ,respectively .In the determination of the reducing ability of barley spent gains polyphenols ,w hen the absorbance was at 0 .6 ,the mass concentration of catechin ,vitamin C ,essence extract and crude extract were 48 .8 ,54 .8 ,44 .1 and 72 .9 μg/ml ,respectively .The essence extract of polyphenol from barley spent gains was higher than catechin ,vitamin C and crude extract in vitro‐antioxidative activity .
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