OBJECTIVE:To study the effects and mechanism of microcystin LR(MC-LR)on oxidative damage and apoptosis in rat ovarian granulosa cells. METHODS:After 48 h exposure to MC-LR at 0,10,20 and 30μg/mL,the level of intracelluar reactive oxygen species, superoxide dismutase (T-SOD) activity and MDA in culture medium,apoptosis and the mRNA changes of Bax and Bcl-2 expressions were evaluated. RESULTS:After MC-LR exposure at the dosage of 10,20 and 30μg/mL for 48 h,the T-SOD activity was significantly suppressed (P<0.05). The concentrations of ROS and MDA in the 20 μg/mL and 30μg/ml groups were significantly reduced (P<0.05) when compared with control group. The correlation coefficient between dosage of MC-LR and ROS was 0.925(P<0.01),between dosage of MC-LR and MDA was 0.811(P<0.01),between dosage of MC-LR and T-SOD was-0.865(P<0.05). Cell assay indicated that the expression of Bax was up-regulated(P<0.05) and Bcl-2 down- regulated (P<0.05). The correlation coefficient between dosage of MC-LR and Bax was 0.972(P<0.01),between dosage of MC-LR and Bcl-2 was -0.972 (P<0.01). CONCLUSION:MC-LR was capable of inducing oxidative damage of rat granulosa cells in vitro. MC-LR could cause apoptosis and changes in the expresons of Bax and Bcl-2.%目的：研究微囊藻毒素LR(MC-LR)对大鼠卵巢颗粒细胞的氧化损伤和致凋亡作用。方法：分别以10、20、30µg/mL的MC-LR对体外培养的大鼠卵巢颗粒细胞染毒48 h，测定细胞内活性氧(ROS)含量、细胞培养液超氧化物歧化酶(T-SOD)活性、丙二醛(MDA)含量、卵巢颗粒细胞凋亡率和凋亡相关基因Bax、Bcl-2 mRNA的表达水平。结果：与对照组比较，随着MC-LR剂量升高，染毒组细胞内ROS升高(rs=0.925，<0.01)；细胞培养液中T-SOD活性降低( s=-0.865，P<0.05)；MDA含量升高(rs=0.811，P<0.01)。卵巢颗粒细胞凋亡率随MC-LR剂量的升高而升高(P<0.05)；凋亡相关基因Bax mRNA的表达量随MC-LR剂量的升高而升高(rs=0.972，P<0.01)，但Bcl-2 mRNA的表达量随MC-LR剂量的升高而降低(rs=-0.972，P<0.01)。结论：MC-LR对大鼠卵巢颗粒细胞具有氧化损伤作用，并能引起卵巢颗粒细胞凋亡和凋亡相关基因Bax、Bcl-2 mRNA表达的改变。P r
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