采用6个微卫星位点研究6个绵羊品种遗传多样性

摘要

利用6个微卫星座位对中国乌珠穆沁羊、小尾寒羊、滩羊、昭通绵羊、呼伦贝尔羊和甘肃高山细毛羊6个绵羊品种，共计280个个体进行遗传多样性分析。计算了6个绵羊品种间的多态信息含量、杂合度和遗传距离，并进行了主成分分析和UPGMA聚类。发现了101个等位基因，平均杂合度0.599-0.691，平均多态信息含量0.609-0.680；甘肃高山细毛羊与其他绵羊品种遗传分歧最大，而呼伦贝尔羊与乌珠穆沁羊间的分歧最小。结果表明6个绵羊品种均具有较高的遗传多样性，品种间的遗传关系与其形成历史、分化及地理分布基本一致。%Six microsatellite loci were used to analyze the genetic diversity of 280 sheep from 6 Chinese breeds of Ujumqin sheep,Small-tailed Han sheep,Tan sheep,Zhaotong sheep,Hulun Buir sheep,and Gansu Alpine Merino. We calculated the polymorphism information content,heterozygosity and genetic distance,analyzed the principal components and clustered UPGMA from these 6 sheep breeds. Altogether, 101 alleles were found,the mean heterozygosity was 0.599-0.691,and mean polymorphism information content was 0.609-0.680. The Gansu Alpine Merino sheep had the most genetic divergence compared to other sheep breeds,while the Hulun Buir and Ujumqin ones were the least in genetic divergence. The above results showed that these 6 breeds had relatively high genetic diversity,and their genetic relationships were consistent with their formation history,differentiation and geographical distribution.