Aim To prepare for tea polyphenols phytosomes and study its antioxidant properties to make a reference to the actual production Methods The difference of characteristic absorption peak,oil/water partition coefficient (K) was investigated to evaluate the preparation of TP-ph. Also we observed its antioxidant properties by POV.Results It shows TP-ph decreased significantly the characteristic absorption peak of TP. In two kind of liposoluble organic solvent,the oil/water partition coefficient of TP-ph is 27.4 and 115.9 when the TP is 4 and 0.00037.Antioxidant index shows refer of TP-ph and TP,the inhibition rate to DPPH would be 50% and 94.1%.For per unit mass at the end of 78 hours,98℃,the POV of T80-ph,rosemary extract,T80 and Ve is 106.67,165.3,200.3 and 202.8.Conclusion Tea polyphenols phytosomes is a feasible approach and it improves significantly on lipid solubility and antioxidant index with TP.%目的 制备茶多酚磷脂复合物(TP-ph),考察它的脂溶性和脂质过氧化性能,为实际应用提供依据.方法 以茶多酚与茶多酚磷脂复合物在茶多酚特征吸收峰位置的吸收值差;以表观油水分配系数K值的测定考察茶多酚磷脂复合物包埋构成及完整性.考察对二苯代苦味肼自由基的抑制率;抗油脂脂质过氧化性能的测定考察比较茶多酚磷脂复合物的抗氧化性能.结果 包埋构成及完整性指标显示茶多酚磷脂复合物显著降低了茶多酚在其特征峰位置的吸收值.茶多酚磷脂复合物与茶多酚的表观油水分配系数在两种脂溶性有机溶剂中的K值分别为27.4和4及115.9和0.00037.抗氧化指标考察显示茶多酚磷脂复合物和茶多酚对二苯代苦味肼自由基的单位质量抑制率分别为50%和94.1%.单位质量抗POV性能方面,在98℃,78 h末,T80-ph、迷迭香提取物、T80 和Ve的POV值分别为106.67、165.3、200.3和202.8.结论 用磷脂包埋茶多酚从构成和完整性指标看是切实可行的,茶多酚磷脂复合物显著增加了茶多酚的脂溶性.且茶多酚磷脂复合物显著增加了茶多酚的抗脂质过氧化性能.
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