没药甾酮对胆管癌 RBE 细胞移植瘤生长和 miRNA表达谱的影响

摘要

目的：研究没药甾酮对胆管癌RBE细胞株移植瘤生长和miRNA表达的影响。方法雌性SD大鼠建立胆管癌RBE细胞株移植瘤模型，随机分为对照组和没药甾酮高、低剂量实验组；高、低剂量实验组大鼠分别予40、20 mg· kg－1· d－1没药甾酮灌胃，对照组大鼠予生理盐水灌胃。每5天测量裸鼠质量和肿瘤体积，处理30 d后取材，提取肿瘤组织总RNA并分离miR-NA。采用微阵列杂交，通过芯片扫描和数据分析获得miRNAs表达谱。结果高、低剂量实验组大鼠移植瘤体积分别在处理20 d和25 d后显著低于对照组（P＜0．01）；处理30 d 后高、低剂量组移植瘤质量均显著低于对照组（P＜0．01）。芯片结果显示，高剂量实验组瘤组织miRNAs表达谱与对照组相比有显著差异。结论没药甾酮可以显著抑制胆管癌RBE细胞株移植瘤生长并影响其miRNA的表达。%Objective To determine the effects of guggulsterone on the growth and microRNAs expression profile in transplanted tumor of human cholangiocarcinoma RBE cells.Methods Female SD nude mice were injected with RBE cells to establish transplanted tumor models.The mice were equally randomized into 3 groups including control group,high dose and low dose treatment groups.Oral admin-istration of guggulsterone [0, 40 and 20 mg· kg-1 · d-1 respectively] were given to the corresponding mice in 72 h after transplanta-tion.Tumor volumes and weights were measured every 5 d.Mice were sacrificed after 30 days’ treatment.Total RNA was extracted and miRNAs were isolated and hybridized with miRNAs microarrays.Results The growth of transplanted tumors was significantly sup-pressed in treatment groups.Tumors volume were significantly smaller in high dose group and low dose group than in the control after 20 days’(P<0.01) and 25 days’treatment (P <0.01).The microarray assay showed significantly different miRNAs expression in tumors of high dose group compared with the control group Conclusion Guggulsterone significantly suppresses the growth of transplan-ted tumor of human cholangiocarcinoma RBE cells and affect microRNAs expression profile.