猪圆环病毒2型特异性单克隆抗体的制备与鉴定

摘要

BALB/c mice were immunized using synthetic tandem polypeptide of Cap protein epitope of porcine circovirus type 2 (PCV2) as the antigen. By using lym-phocyte hybridoma technique, a hybridoma cellline stably secreting monoclonal an-tibody against PCV2-rCap protein was successful y obtained and named as 670#. The ascites titer of the obtained monoclonal antibody was 1∶100 000. Western blot results showed that the monoclonal antibody could react with prokaryotical y ex-pressed PET32a-ORF2 recombinant protein, eukaryotical y expressed ORF1-ORF2 tandem protein and PCV2 whole virus celllysate. Indirect EILSA demonstrated that the monoclonal antibody could bind with ORF1-ORF2 tandem protein. Indirect im-munofluorescence assay (IFA) indicated that the monoclonal antibody could identify native PCV2 virus. The preparation of this monoclonal antibody provided technical tools for epitope analysis and molecular diagnosis of PCV2 virus.%以人工合成 PCV2 Cap蛋白表位的串联多肽作为抗原免疫 BALB/c小鼠，利用淋巴细胞瘤杂交技术获得了1株稳定分泌抗PCV2-rCap 蛋白的杂交瘤细胞株，命名为670#。该株杂交瘤细胞诱导同品系小鼠产生的腹水抗体效价为l∶100000。 Western blot结果显示，该株单抗可与表达原核 PCV2 PET32a-ORF2重组蛋白、真核表达 ORF1-ORF2串联蛋白及 PCV2全病毒细胞培养物反应；间接 ELISA证明该单核可与 ORF1-ORF2串联蛋白结合；IFA结果表明，该单抗可与天然PCV2病毒结合。该单抗的制备为 PCV2抗原表位分析及分子诊断提供了技术手段。